Coding
PBSII-Zif2

Part:BBa_K4674004:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


PBSII-Zif268 fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part is codon optimized for expression in E. Coli.


Source

The Zif268 is originated from the eukaryotic EGR1 gene. The PBSII is a synthetic zinc finger protein for recognizing specific DNA motif with high affinity.

References

Conrado RJ, et. al. DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency Nucleic Acids Research, 2012, Vol. 40, No. 4 1879–1889